CELL VIABILITY AND DNA DAMAGE IN MRC5 AND HeLa CELL LINES AFTER HISTONE H1 KNOCKOUT BY CRISPR-Cas9 GENOME EDITING TECHNOLOGY

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Submitted: 2025-03-05; Published: 2025-03-05

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Abstract

Chromatin research mainly focused on the core histones, whereas the role of H1.5 linker histone is poorly understood. Today CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats associated protein 9) genome editing technology provides an opportunity to analyze functions of different genes introducing targeted loss-of function mutations. Here we demonstrate the role of histone H 1.5 in cell viability and genome integrity in HeLa and MRC5 cells using trypan blue exclusion test and single-cell gel electrophoresis (comet) assay.