EFFECT OF DNA GC-CONTENT ON INTERACTION WITH METHYLENE BLUE

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Submitted: 2025-03-10; Published: 2025-03-10

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Abstract

The melting of methylene blue (MB) complexes of DNA with different GC-content sequences at 0.02 M ionic strength of the solution has been carried out. It was shown that the change of melting interval width of MB complexes with DNA increases depending on GC-content enhancement within DNA sequences. It was revealed that MB is bound to DNA by semi-intercalation mode.