IMMOBILIZATION OF RECOMBINANT L-AMINOACYLASE FROM GEOBACILLUS STEAROTERMOPHILUS AND CHARACTERISTICS OF OBTAINED PREPARATIONS

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Submitted: 2025-03-12; Published: 2025-03-12

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Abstract

Thermophilic L-aminoacylase Geobacillus stearotermophiluswas immobilized on silochrome C-80 with glutaraldehyde. Immobilization process does not affect the temperature optima of derived preparations, but increase in the thermal stability of the immobilized aminoacylase was observed. Michaelis constants (Km)were calculated for N-acetyl-L-methionine, N-acetyl-L-valine and N-acetyl-L-alanine. It was shown that as a result of immobilization Km for N-acetyl-L-methionine increased more than 2-fold