COMPARATIVE EVALUATION OF ANTI MICROBIAL EFFICACY USING MANUAL, SINGLE FILE AND MULTIPLE FILE SYSTEM DURING ROOT CANAL PREPARATION IN PRIMARY MOLARS – A RANDOMISED EX VIVO STUDY

Background: Pulpectomy is a key procedure in pediatric dentistry to remove infected pulp from primary teeth, aiming
to prevent periapical infections. Success depends on thorough cleaning and disinfection of root canals. Rotary files,
with their efficiency and reduced procedural time, are increasingly preferred over manual instruments. This study
evaluates the antimicrobial efficacy of manual H-files, Kedo SG Blue, Kedo Nano Plus, and AF Baby rotary file
systems during root canal preparation in primary molars.
Materials and Methods: A randomized ex vivo study was conducted with 45 children aged 4-9 years requiring
pulpectomy in primary molars. Participants were randomly divided into four groups: Group I (Manual H-files), Group
II (Kedo SG Blue), Group III (Kedo Nano Plus), and Group IV (AF Baby). Pre- and post-instrumentation microbial
samples were collected and cultured teasure c). Data were analyzed using the Wilcoxon test and one-way ANOVA.
Results: All groups showed a significant reduction in microbial load post-instrumentation (P < 0.05). Group II (Kedo
SG Blue) had the highest microbial reduction (95%), followed by Group III (92%), Group IV (89%), and Group I
(87%). However, the differences between groups were not statistically significant (P > 0.05).
Conclusion:Kedo-SG Blue demonstrated the greatest antimicrobial efficacy, suggesting that rotary file systems may be
more efficient than manual methods for root canal disinfection in primary teeth.