FABRICATION OF TRIPLE ANTIBIOTIC AND CISSUS QUADRANGULARIS CONJUGATED LYOPHILIZED GUIDED TISSUE REGENERATION MEMBRANE AND ITS CHARACTERISATION

Background: Periodontitis is a severe gum infection that damages the soft tissue and, if untreated, can destroy the
bone that supports your teeth.Cissus quadrangularis, a medicinal plant known for its bone healing properties, has
been used in traditional medicine for centuries. Antibiotic-laden membranes have gained significant attention due
to their ability to prevent infections, which are a major challenge in orthopedic surgeries and bone grafting
procedures. The main aim of the study is to fabricate and characterize the triple antibiotic and Cissus
quadrangularis conjugated lyophilized membrane for periodontal bone regeneration.
Materials and Methods: 10mg of Cissus quadrangularis was weighed and dispersed in 100 ml of deionised waste
and heated at 50 degree centigrade for 24 hrs then the extract was collected using filtration method with wattmann
filter paper. 1 g of PEG and Carrageenan (2 g) was dissolved in 100 mL of cissus quadrangularis extract at 60 ◦C
using magnetic stirrer under 400 rpm . Triple antibiotics were also added. Following a 24-hour period, the petri
plate was dried for 12 hours at 50°C. The membrane was then removed from the petri dish and placed in a
desiccator. Following membrane formation FTIR, contact angle, Antimicrobial activity were compared between
Cissus quadrangularis membrane and carrageenan (control group) and Group 2 consisted of Cissus quadrangularis
membrane, triple antibiotic, and carrageenan (test group).
Results: Tensile strength of the control group is 14.62 N and Test group is 32.13 N. Hence the control group
membrane has more tensile strength than the test group membrane. The Test group membrane showed an contact
angle average of 75.27 degree and Control group membrane showed an contact angle average of 46.51degree.
Hence the control group membrane indicates the better hydrophilic nature of the material. The control group
membrane showed better antimicrobial activity than the test group membrane against the tested bacteria
(Streptococcus mutans).
Conclusion: The present study results showed a higher tensile strength, good antimicrobial property and enhanced
wettability in the prepared novel membrane when compared to the control group. By addressing both the
prevention of infection and the promotion of bone healing, this novel membrane builds on existing research and
offers a promising solution for use in guided tissue regeneration.