Natural Sciences, Stomotology, 2026
ISSN: 1829-006X
DECODING AUTISM:THE SCIENTIFIC LINK BETWEEN SALIVARY VITAMIN D3 LEVELS AND NEURODEVELOPMENT-A CASE-CONTROL STUDY
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Submitted: 2026-04-08
© 2026 by author(s) and The Gufo Inc.
This work is licensed under Creative Commons Attribution–NonCommercial International License
(CC BY-NC 4.0).
Abstract
Objective: Autism Spectrum Disorder (ASD) involves challenges in social communication and repetitive
behaviors. Emerging evidence suggests a link between vitamin D3, vital for brain function, and ASD, though
research on salivary vitamin D3 as a biomarker in ASD is limited.
Aim:To compare salivary vitamin D3 levels between ASD patients and neurotypical individuals.
Materials and Methods: This case-control study collected unstimulated saliva samples from ASD patients and
neurotypical controls. Vitamin D3 levels were measured using enzyme-linked immunosorbent assay (ELISA), with
paired t-tests for group comparisons.
Results: ASD patients had significantly lower salivary vitamin D3 levels than controls (p = 0.001), with ranges of
28.9–48.4 ng/mL in ASD versus 45.2–60.9 ng/mL in neurotypical individuals.
Conclusion:ASD patients showed lower salivary vitamin D3, suggesting a potential association with vitamin D3
deficiency. Further research could explore the role of vitamin D3 in ASD and assess the benefits of monitoring and
supplementation.