Clinical and experimental validat ion of the ozone therapy effectiveness in case of accidental exposure of the dental pulp

An experimental study was conducted on rabbits of the “Soviet chinchilla” breed. Iatrogenic pulpitis was modeled in animals, in the first group only a direct coating with the material “Trioxident” was used, 12 animals, in the second group, 12 animals, before covering the pulp with the material “Trioxident”, the opened tooth pulp was blown with an ozone-air mixture. A study was conducted in 18 patients with an accidental opening of the pulp of the tooth aged 20 to 45 years. The first group consisted of 9 patients, who were treated with ozone therapy in order to preserve the viability of the pulp, after the pulp was directly coated with “Trioxident” material.
In the second group of 9 patients ozone therapy was not prescribed, but a direct coating of the pulp with “Trioxident” material was also performed. The experiment revealed that ozone therapy combined with direct coating of the pulp with material “Trioxident” has anti-inflammatory effect on the structure of the tooth pulp, and also stimulates dentin formation and regeneration of the pulp. Macrophage and the intensity of reaction with S100 on the 14th day of treatment of iatrogenic pulpitis and formation of thin, high-density dentin bridges and structuring odontoblasts in a single layer on the 35th day.
The result of studying the effectiveness of ozone therapy in case of accidental opening
of the tooth pulp in clinical practice proved that in the group where ozone therapy was not used, two of nine patients had irreversible pathological changes in the pulp. In the group where ozone therapy was used, and after direct coating of the pulp with “Trioxident” material was performed, all patients managed to preserve the viability of the pulp.