ACTH-DEPENDENT PROCESS OF LIPASE EXCRETION BY RAT PANCREATIC ACINAR CELLS

Modulator effect of adrenocorticotropic hormone (ACTH) on the process of lipase excretion by rat pancreatic acinar cells was studied in the model in vivo and in vitro experiments. Before the experiment an investigation protocol was drawn up which included all necessary conditions and phases mandatory to study the impact of endogenously active substances of hormonal nature upon diffrent integrative systems of the mammal organism. The protocol included a number of cumpalsory conditions, which we believe should be followed by the researchers while studying different
aspects of endocrine system. It’s about corticosterone and transcortin, released concretely in rats, which are endowed with circadian and seasonal rhythm. Synacthen (Germany), a synthetic adrenocorticotropic hormone analog, was used in the trial. Lipase activity was determined by means of diagnostic Lipase Activity Assay Kit (Spain). As revealed in in vivo experiment i.e. intravenous infusion of Synachten in a dose of 6x105 mol/L, the first peak of lipase activity in serum was observed in the 10-15th minutes while the second peak occured in the 20-30th minutes.
During in vitro experiments (in the conditions of bile duct drainage with Synachten introduction) lipase activity peak in pancreas was determined in 20th minute. During in vitro experiments (on the pancreatic cell culture in the conditions of Synacthen introduction into the cultural environment) release of lipase from acinar cells was observed at quite a wide range of injected hormone concentration -10-10- 10-15Ml. The conducted researches found out that synthetic adrenocorticotropic hormone analog in therapeutic dose under both in vivo and in vitro conditions produces stimulating effect on the process of lipase release by the cells. Due to in vitro studies (on the pancreatic cell culture) it was determined that synthetic ACTH analog in a dose a magnitude higher
than therapeutic dose, i.e. 10 -15mol provides a significant release from the cultured cells.
Thus, in the experiment conducted on rats, a new ‘side’ effect of adrenocorticotropic hormone was revealed, in which excretory apparatus of the pancreas with enhanced release of lipase by acinar cells presents as a target.