Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 66–72
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ASSESSING TUMOR MICROENVIRONMENT OF ORAL EPITHELIAL DYSPLASIA AND ORAL SQUAMOUS CELL CARCINOMA IN ORAL SUBMUCOUS FIBROSIS USING MASSON’S TRICHROME

Vimalasubhashini Vivekbalamithran*, Abilasha Ramasubramanian, Hanif Sulistya

Oral Pathology and Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai-600077, Tamil Nadu, India. · Faculty of Medicine, Airlangga University, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

Received: 2026-04-08 · Published: 2025-06-15

© 2025 The Author(s) and the original publisher.

CC BY-NC 4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Original title
ASSESSING TUMOR MICROENVIRONMENT OF ORAL EPITHELIAL DYSPLASIA AND ORAL SQUAMOUS CELL CARCINOMA IN ORAL SUBMUCOUS FIBROSIS USING MASSON’S TRICHROME
Authors
Vimalasubhashini Vivekbalamithran, Abilasha Ramasubramanian, Hanif Sulistya
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-06-15
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.21.5-66

Abstract

Background: Oral submucous fibrosis (OSMF) is a chronic, progressive disease that affects both epithelial and connective tissue structures. Grading according to various histological parameters which include the epithelial and the connective tissue features can be demonstrated in routine hematoxylin and eosin stains(H&E). However, the use of special stains ensures better visualization which aids in early diagnosis of these connective tissue changes which includes microenvironmental changes and epithelial mesenchymal transition especially in advanced OSMF cases. The present study aims to compare the micro environmental changes and epithelial mesenchymal transition by evaluation of muscle involvement and degeneration in cases with advanced oral submucous fibrosis using Masson’s trichrome Methods: Grouping: Group 1: OED with OSMF (5 cases) Group 2: OSCC with OSMF (5 cases) Total 10 cases were taken and stained with both H&E staining and Masson’s trichrome stain. This helps in assessing tumor microenvironment of oral epithelial dysplasia and oral squamous cell carcinoma in Oral submucous fibrosis using Masson’s trichrome. Results: This study shows higher amounts of inflammation in OSMF with the OSCC group, as it can be easily differentiated and helps in identifying malignant transformation. Among the two groups, oral epithelial dysplasia with Oral submucous fibrosis shows more fibrosis extension into the muscle. Conclusion: The questionable areas of degenerating muscle bundles or areas of hyalinization were better distinguished with Masson’s stain, especially in deeper connective tissue and was statistically significant. The purpose of this study is to identify potentially malignant diseases from malignant transformation of dysplasia and helps to study how collagen deposition can affect oral squamous cell carcinoma.
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