Natural Sciences, Stomotology, 2026
ISSN: 1829-006X
COMPARATIVE ANALYSIS OF HERBAL ROOT CANAL IRRIGANT’S ANTIBACTERIAL EFFECTIVENESS
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Submitted: 2026-04-08
© 2026 by author(s) and The Gufo Inc.
This work is licensed under Creative Commons Attribution–NonCommercial International License
(CC BY-NC 4.0).
Abstract
Background: The total removal of microorganisms from the root canal is essential for effective root canal procedure.
The objective of this research was to assess the antibacterial properties of sodium hypochlorite (NaOCl) in
combination with herbal root canal irrigants (Tulsi, neem extract, and triphala).
Methods: Mueller-Hinton agar plates were injected with the bacterial E. faecalis(ATCC) culture, which had been
cultured overnight in brain heart infusion (BHI) broth. The agar well diffusion method was used to measure
antibacterial inhibition. After adding each of the four research irrigants to its corresponding well on an agar plate, the
plates were incubated for 24 hrs at 37°C. Each well’s surrounding bacterial inhibitory zone was observed. The findings
were examined statistically.
Result: Neem and triphala extracts exhibited the greater inhibitory zone against E. faecalis in NaOCl, whereas tulsi
extract showed the least.
Conclusion: Neem extract, triphala, and tulsi, three herbal remedies that were tested, shown an inhibitory zone against E. faecalis. Therefore, these irrigants can be applied as irrigating solutions for root canals.