COMPARATIVE EVALUATION OF SIX INTERMEDIATE ROOT CANAL IRRIGANTS TO PREVENT ORANGE-BROWN PRECIPITATE FORMATION DUE TO INTERACTION OF SODIUM HYPOCHLORITE AND CHLORHEXIDINE GLUCONAT

Aim: In-vitro comparative evaluation of Six Intermediate root canal irrigants to prevent Orange-Brown colour
precipitate formation due to Interaction of Sodium hypochlorite and Chlorhexidine gluconate solutions during
endodontic treatment using Stereomicroscope analysis.
Materials & Method: Seventy human extracted permanent mandibular single rooted premolar teeth were collected.
Access cavity preparation done, working length was determined and biomechanical preparation was done using
ProTaper Next rotary files upto size X3 file. All specimens were irrigated with 2ml of 3% NaOCl solution and equally
divided into Seven groups with 10 specimens each, based on type of Intermediate canal irrigants used. Group 1: 0.9%
Normal Saline, Group 2: Distilled water at room temperature, Group 3: Distilled water at 65C, Group 4: 20% Citric
acid, Group 5: 70% Isopropyl alcohol, Group 6: 0.2% Chitosan solution, Group 7: Control group (No intermediate canal
irrigants used). All specimens were then finally irrigated with 2ml of 2% Chlorhexine gluconate solution. All specimens
were then longitudinally sectioned into two equal halves and each halve was examined at 9mm (coronal), 6mm (middle)
& 3mm (apical) third’s of root canals with Stereomicroscope for presence or absence of Orange-Brown colour
Precipitate formation.
Statistical plan: OneWay ANOVA and Tukey Post-hoc tests were used for statistical analysis. P ≤0.05 was
considered statistically significant.
Results: Statistically highly significant difference (P<0.05) was seen in inter-group comparison among the groups.
Group 5 (70% Isopropyl alcohol) showed maximum efficiency in the prevention of orange-brown precipitate
formation followed by Group 6 (0.2% Chitosan solution) both at coronal, middle and apical-third’s of root canals.
Conclusion: 70% Isopropyl alcohol used as Intermediate canal irrigant between the use of sodium hypochlorite and
Chlorhexidine solutions showed maximum efficiency in preventing the formation of orange-brown precipitate
compared to other Intermediate canal irrigants used at all 3-levels of root canals.