Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 162–172
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COMPARATIVE EVALUATION OF SIX INTERMEDIATE ROOT CANAL IRRIGANTS TO PREVENT ORANGE-BROWN PRECIPITATE FORMATION DUE TO INTERACTION OF SODIUM HYPOCHLORITE AND CHLORHEXIDINE GLUCONAT

Received: 2026-04-08 · Published: 2025-11-05

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Original title
COMPARATIVE EVALUATION OF SIX INTERMEDIATE ROOT CANAL IRRIGANTS TO PREVENT ORANGE-BROWN PRECIPITATE FORMATION DUE TO INTERACTION OF SODIUM HYPOCHLORITE AND CHLORHEXIDINE GLUCONAT
Author
Srikumar Srikumar
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-11-05
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.21.10-162

Abstract

Aim: In-vitro comparative evaluation of Six Intermediate root canal irrigants to prevent Orange-Brown colour precipitate formation due to Interaction of Sodium hypochlorite and Chlorhexidine gluconate solutions during endodontic treatment using Stereomicroscope analysis. Materials & Method: Seventy human extracted permanent mandibular single rooted premolar teeth were collected. Access cavity preparation done, working length was determined and biomechanical preparation was done using ProTaper Next rotary files upto size X3 file. All specimens were irrigated with 2ml of 3% NaOCl solution and equally divided into Seven groups with 10 specimens each, based on type of Intermediate canal irrigants used. Group 1: 0.9% Normal Saline, Group 2: Distilled water at room temperature, Group 3: Distilled water at 65C, Group 4: 20% Citric acid, Group 5: 70% Isopropyl alcohol, Group 6: 0.2% Chitosan solution, Group 7: Control group (No intermediate canal irrigants used). All specimens were then finally irrigated with 2ml of 2% Chlorhexine gluconate solution. All specimens were then longitudinally sectioned into two equal halves and each halve was examined at 9mm (coronal), 6mm (middle) & 3mm (apical) third’s of root canals with Stereomicroscope for presence or absence of Orange-Brown colour Precipitate formation. Statistical plan: OneWay ANOVA and Tukey Post-hoc tests were used for statistical analysis. P ≤0.05 was considered statistically significant. Results: Statistically highly significant difference (P<0.05) was seen in inter-group comparison among the groups. Group 5 (70% Isopropyl alcohol) showed maximum efficiency in the prevention of orange-brown precipitate formation followed by Group 6 (0.2% Chitosan solution) both at coronal, middle and apical-third’s of root canals. Conclusion: 70% Isopropyl alcohol used as Intermediate canal irrigant between the use of sodium hypochlorite and Chlorhexidine solutions showed maximum efficiency in preventing the formation of orange-brown precipitate compared to other Intermediate canal irrigants used at all 3-levels of root canals.
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