FABRICATION AND CHARACTERIZATION OF VITAMIN C CONJUGATED CISSUS QUADRANGULARIS MEMBRANE FOR GUIDED TISSUE REGENERATION – AN IN VITRO STUDY

Background: Cissus quadrangularis has gained attention for its rich content of bioactive compounds, including
flavonoids, carotenoids, and polyphenols, notably Vitamin C.These compounds are recognized for their antioxidant,
anti-inflammatory, and tissue reparative properties, may provide the membrane more bioactivity, which would
encourage cell division and proliferation.This main aim of this study is the production of vitamin C membrane
impregnated with Cissus quadrangularis for soft tissue regeneration.
Materials and Methods: To prepare the vitamin C membrane with the impregnation of Cissus quadrangularis (CQ)
, 1 gram of CQ was dissolved in 10 mL of distilled water and heated at 80°C for 24 hours. Following membrane
formation, FTIR (Fourier Transform InfraRed Spectroscopy), contact angle, tensile strength testing and antimicrobial
activity was compared between the CAR control group membrane and the test group membrane which contains CAR
with the impregnation of vitamin C.
Results: The tensile strength of the test group was better than the control group. The test group showed the
tensile strength of 23.86 N whereas the control group showed 27.99 N. CAR membrane had a contact angle
of 81.88 degrees. This implies lower wettability. Whereas the cissus quadrangularis and vitamin C
incorporated membrane had a contact angle of 49.92 degrees. The test group showed better wettability,
tensile strength and anti microbial activity as compared to the control group.
Conclusions: The present study showed that CAR membranes with the impregnation of Cissus
quadrangularis showed increase in hydrophilicity. The mechanical property was increased and the
antimicrobial activity showed an enhanced zone of inhibition. The membrane can serve multipurpose dental
applications and can be incorporated in various periodontal procedures for guided tissue regeneration.