In many clinical disorders and cancers, immunohistochemistry (IHC) is employed extensively for
diagnosis and prognosis. This study was conducted to evaluate the impact of fixation time on Ki67
immunohistochemical expression in rat oral tissues biopsies. 20 albino Rats were employed. The palatal
oral mucosa, both soft and hard, was dissected and preserved for one day and seven days in 10% neutral
buffered formalin. A Ki67 immunohistochemical staining procedure was used. The findings were
statistically analyzed using SPSS after the percentages of positively stained nuclei were graded. Using 10%
NBF, there were no significant differences in Ki67 expression between the soft and hard palatal epithelium
of rat oral cavity after one day or seven days of fixation. However, there were significant differences
between the soft and hard palate specimens after one day and seven days of fixation. As a conclusion , the
fixation time within 1 to 7 days has no effect on Ki67 staining of both studied oral sites. On the other hand,
Ki67 expression is slightly higher in hard palate epithelium than soft palate regardless of the fixation time
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