PREPARATION AND CHARACTERIZATION OF HYDROXYAPATITE AND CISSUS QUADRANGULARIS COMPOSITE ENTRAPPED HYDROXYPROPYL METHYLCELLULOSE SCAFFOLD FOR BONE REGENERATION-AN INVITRO STUDY

Background: The integration of biomaterials, such as hydroxyapatite (HAp) and natural extracts like Cissus
quadrangularis (CQ), into scaffolds can enhance osteogenic properties and support bone healing. This study
evaluates the potential of an HAp-CQ composite embedded in a Hydroxypropyl methylcellulose (HPMC) scaffold
for bone regeneration.
Material and methods: The HAp-CQ composite was synthesized using a sol-gel method. HPMC scaffolds were
prepared through a solvent-casting and particulate-leaching technique, followed by the incorporation of the HApCQ composite. To prepare the hydroxypropyl methylcellulose scaffold with the impregnation of Cissus
quadrangularis (CQ) and hydroxyapatite (HAp), 1 gram of CQ was dissolved in 10 mL of distilled water and
heated at 80°C for 24 hours. Following scaffold formation, FTIR (Fourier Transform Infrared Spectroscopy),
contact angle, tensile strength testing and anti-microbial activity was compared between the HAp-CQ and
HPMC+HAp+CQ scaffold.
Results:The synthesized HAp-CQ composite displayed improved mechanical properties and bioactivity, with
FTIR confirming functional group interactions. The tensile strength of the test group scaffold was 1.51MPa and
the contact angle of the test group scaffold showed an average angle of 53.07 degrees.
Conclusion: The present study showed that the HPMC scaffold with the impregnation of CQ and HAp showed
high tensile strength and increase in hydrophilicity. The mechanical property was increased and the antimicrobial
activity showed an enhanced zone of inhibition.