ADENOSINE DEAMINASE AND GLUTAMINASE IN DUPUYTREN’S CONTRACTURE COMBINED WITH DIABETES MELLITUS

The etiology of Dupuytren's contracture (DC), a disease characterized by the thickening of the palmar aponeurosis, remains relatively understudied. Diabetes mellitus (DM) is identified as one of the risk factors for DC. The objective of the present research is to find out the relation of DM to the DC in the level of some essential enzymes. The object of the study was the hand's aponeurosis. The activities of adenosine deaminase (ADA) and glutaminase (GLS) enzymes were studied in the aponeurosis tissue homogenate. 15 individuals participated in the research and were divided into 3 groups per 5 persons: the group of individuals with only DC, the group consisted of individuals with DC and DM together (DC+DM), and the group of the relatively healthy subjects served as a control. The activity of GLS in the control group was equal to 0.92 ± 0.0180 IU/µg protein, and in the DC and DC+DM groups decreased down to 0.60 ± 0.03 IU/µg protein and 0.12 ± 0.012 IU/µg protein, relatively. On the contrary, the activity of ADA was in the control group of 0.057 ± 0.007 IU/µg protein and increased in the DC and DC+DM groups up to 0.47 ± 0.03 IU/µg protein and 0.94 ± 0.062 IU/µg protein, respectively. The results of the research indicated that these two enzymes are associated with the ethology and pathogenesis of DC development.