2015; Chemical and Biological Sciences 49 (3 (238): 29–34
Shared with The Gufo

BINDING MECHANISMS OF METHYLENE BLUE AND HOECHST 33258 WITH DNA

Ara Antonyan*

Received: 2025-03-10 · Published: 2015-11-20

© 2015 The Author(s) and the original publisher.

Licence: as stated by the publisher.
Shared article.
Original title
BINDING MECHANISMS OF METHYLENE BLUE AND HOECHST 33258 WITH DNA
Author
Ara Antonyan
Published
2015-11-20
Licence
as stated by the publisher

Abstract

In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It was shown that Hoechst 33258 was bound to DNA by two types – “strong” and “weak”. It was also shown that the “strong” type of Hoechst 33258 binding depends on solution ionic strength.
1 / ? 100% Open in new tab Download Cite

Loading the full text…

Download Follow Updates