2016; Chemical and Biological Sciences 50 (3 (241): 64–66
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CELL VIABILITY AND DNA DAMAGE IN MRC5 AND HeLa CELL LINES AFTER HISTONE H1 KNOCKOUT BY CRISPR-Cas9 GENOME EDITING TECHNOLOGY

Tigran Harutyunyan*

Received: 2025-03-05 · Published: 2016-11-21

© 2016 The Author(s) and the original publisher.

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Original title
CELL VIABILITY AND DNA DAMAGE IN MRC5 AND HeLa CELL LINES AFTER HISTONE H1 KNOCKOUT BY CRISPR-Cas9 GENOME EDITING TECHNOLOGY
Author
Tigran Harutyunyan
Published
2016-11-21
Licence
as stated by the publisher

Abstract

Chromatin research mainly focused on the core histones, whereas the role of H1.5 linker histone is poorly understood. Today CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats associated protein 9) genome editing technology provides an opportunity to analyze functions of different genes introducing targeted loss-of function mutations. Here we demonstrate the role of histone H 1.5 in cell viability and genome integrity in HeLa and MRC5 cells using trypan blue exclusion test and single-cell gel electrophoresis (comet) assay.
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