CLINICAL APPLICATIONS AND REGENERATIVE POTENTIAL OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN WOUND HEALING: A:LITERATURE REVIEW

Background: Chronic and acute wounds continue to pose significant clinical and socioeconomic challenges, particularly when complicated by infection, ischemia, or delayed re-epithelialization. Decellularized human amniotic membrane (dHAM) has gained attention as a biological scaffold capable of supporting tissue regeneration due to its intrinsic biocompatibility, anti-inflammatory properties, and structural similarity to native extracellular matrix (ECM).
Objective:This literature review examines the clinical evidence on the safety and effectiveness of dHAM in skin and
soft tissue wound healing, focusing on decellularization methods, scaffold properties, and patient outcomes.
Methods:A systematic search of PubMed, Scopus, and Web of Science was conducted using keywords such as
“decellularized human amnion,” “scaffold,” and “wound healing.” Only human clinical studies involving dHAM were
included; preclinical, animal, and fresh or cryopreserved amnion studies were excluded. Thirteen studies out of 278 met the inclusion criteria for qualitative analysis.
Results: Decellularization via physical, chemical, and enzymatic methods effectively removed cells while preserving
ECM structure and bioactivity. Clinically, dHAM promoted faster epithelialization and angiogenesis, reduced
inflammation, and improved scar quality in various wound types. Most wounds achieved closure within two to four
weeks, with less pain and exudate compared to conventional dressings. Furthermore, modified dHAM scaffolds
containing bioactive agents or hydrogels showed enhanced tissue regeneration and vascularization.
Conclusion: dHAM is a safe, versatile biomaterial that combines structural support with biological activity to accelerate organized wound healing. With standardized processing and additional clinical trials, dHAM holds significant promise as a component of advanced regenerative wound care.