Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 504–510
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COMPARATIVE EVALUATION OF ANTI MICROBIAL EFFICACY USING MANUAL, SINGLE FILE AND MULTIPLE FILE SYSTEM DURING ROOT CANAL PREPARATION IN PRIMARY MOLARS - A RANDOMISED EX VIVO STUDY

Ganesh Jeevanandan*

Received: 2026-04-08 · Published: 2025-10-23

© 2025 The Author(s) and the original publisher.

CC BY-NC 4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Original title
COMPARATIVE EVALUATION OF ANTI MICROBIAL EFFICACY USING MANUAL, SINGLE FILE AND MULTIPLE FILE SYSTEM DURING ROOT CANAL PREPARATION IN PRIMARY MOLARS - A RANDOMISED EX VIVO STUDY
Author
Ganesh Jeevanandan
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-10-23
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.21.9-504

Abstract

Background: Pulpectomy is a key procedure in pediatric dentistry to remove infected pulp from primary teeth, aiming to prevent periapical infections. Success depends on thorough cleaning and disinfection of root canals. Rotary files, with their efficiency and reduced procedural time, are increasingly preferred over manual instruments. This study evaluates the antimicrobial efficacy of manual H-files, Kedo SG Blue, Kedo Nano Plus, and AF Baby rotary file systems during root canal preparation in primary molars. Materials and Methods: A randomized ex vivo study was conducted with 45 children aged 4-9 years requiring pulpectomy in primary molars. Participants were randomly divided into four groups: Group I (Manual H-files), Group II (Kedo SG Blue), Group III (Kedo Nano Plus), and Group IV (AF Baby). Pre- and post-instrumentation microbial samples were collected and cultured teasure c). Data were analyzed using the Wilcoxon test and one-way ANOVA. Results: All groups showed a significant reduction in microbial load post-instrumentation (P < 0.05). Group II (Kedo SG Blue) had the highest microbial reduction (95%), followed by Group III (92%), Group IV (89%), and Group I (87%). However, the differences between groups were not statistically significant (P > 0.05). Conclusion:Kedo-SG Blue demonstrated the greatest antimicrobial efficacy, suggesting that rotary file systems may be more efficient than manual methods for root canal disinfection in primary teeth.
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