Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 359–368
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COMPARATIVE EVALUATION OF AZADIRACHTIN, VITAMIN C AND INSULIN LIKE GROWTH FACTOR 1 RELEASE IN TITANIUM PLATELET RICH FIBRIN INFUSED WITH NEEM AND TRIPHALA INDICA GEL EXTRACTS: AN INVITRO STUDY

Shiva Shankar Gummaluri*

Received: 2026-04-08 · Published: 2025-06-30

© 2025 The Author(s) and the original publisher.

CC BY-NC 4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Original title
COMPARATIVE EVALUATION OF AZADIRACHTIN, VITAMIN C AND INSULIN LIKE GROWTH FACTOR 1 RELEASE IN TITANIUM PLATELET RICH FIBRIN INFUSED WITH NEEM AND TRIPHALA INDICA GEL EXTRACTS: AN INVITRO STUDY
Author
Shiva Shankar Gummaluri
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-06-30
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.21.5-359

Abstract

Background: Herbal extract gel addition to Titanium -Platelet Rich Fibrin (T-PRF) clots was started very recently. Their release from the T-PRF clots has not been performed. Hence present study aimed to evaluate the release of Azadirachtin (Az) from T-PRF+Neem gel group, Vitamin C release from T- PRF+ Triphala Indica group and Insulin like growth factor 1 (IGF 1) from T-PRF alone along with the mentioned test groups. Methods and Materials:Present invitro study utilized 6 subject’s blood and subjected to centrifugation. T-PRF clots were prepared and TI/ NE gel extracts were injected and compared with T-PRF alone to check the release of Vit C, Az and IGF 1 release at 6hours, 72 hours (3rd), 7th and 14th day. For statistical analysis, paired t test and ANOVA was performed. P value <0.05 was considered statistically significant. Results: Az release there was decreased levels reported between 6 hrs and the 7th day and it was statistically significant (p = 0.045*). Whereas, all other time frame comparisons values were non-significant (p>0.05) Regarding Vit C release from T-PRF clot there was a decrease in the amount released when compared at 6hrs & 7th day (p=0.008*) and 72hrs and 7th day (p=0.037*). IGF 1 release in all the three groups and at different time frames was non-significant (p>0.05). Conclusion: Added herbal extracts didn’t alter the release of IGF 1 that proves the surety of GF release. T-PRF also showed the timely release of the Az and Vit C confirming the name of sustained drug delivery system (SDDS).
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