COMPARATIVE EVALUATION OF AZADIRACHTIN, VITAMIN C AND INSULIN LIKE GROWTH FACTOR 1 RELEASE IN TITANIUM PLATELET RICH FIBRIN INFUSED WITH NEEM AND TRIPHALA INDICA GEL EXTRACTS: AN INVITRO STUDY

Background: Herbal extract gel addition to Titanium -Platelet Rich Fibrin (T-PRF) clots was started very recently.
Their release from the T-PRF clots has not been performed. Hence present study aimed to evaluate the release of
Azadirachtin (Az) from T-PRF+Neem gel group, Vitamin C release from T- PRF+ Triphala Indica group and Insulin
like growth factor 1 (IGF 1) from T-PRF alone along with the mentioned test groups.
Methods and Materials:Present invitro study utilized 6 subject’s blood and subjected to centrifugation. T-PRF clots
were prepared and TI/ NE gel extracts were injected and compared with T-PRF alone to check the release of Vit C,
Az and IGF 1 release at 6hours, 72 hours (3rd), 7th and 14th day. For statistical analysis, paired t test and ANOVA was
performed. P value <0.05 was considered statistically significant.
Results: Az release there was decreased levels reported between 6 hrs and the 7th day and it was statistically
significant (p = 0.045*). Whereas, all other time frame comparisons values were non-significant (p>0.05) Regarding
Vit C release from T-PRF clot there was a decrease in the amount released when compared at 6hrs & 7th day
(p=0.008*) and 72hrs and 7th day (p=0.037*). IGF 1 release in all the three groups and at different time frames was
non-significant (p>0.05).
Conclusion: Added herbal extracts didn’t alter the release of IGF 1 that proves the surety of GF release. T-PRF also
showed the timely release of the Az and Vit C confirming the name of sustained drug delivery system (SDDS).