Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 158–169
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COMPARISON OF SERUM CYTOKINES IL-6, TNF-α, AND IL-10 AFTER SUBCUTANEOUS IMPLANTATION OF DECELLULARIZED AND NON DECELLULARIZED FREEZE DRIED BOVINE BONE SCAFFOLD (In vivo laboratory research on Rattus Novergicus)

Kharisma Nisa*

Received: 2026-04-08 · Published: 2025-07-15

© 2025 The Author(s) and the original publisher.

CC BY-NC 4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Original title
COMPARISON OF SERUM CYTOKINES IL-6, TNF-α, AND IL-10 AFTER SUBCUTANEOUS IMPLANTATION OF DECELLULARIZED AND NON DECELLULARIZED FREEZE DRIED BOVINE BONE SCAFFOLD (In vivo laboratory research on Rattus Novergicus)
Author
Kharisma Nisa
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-07-15
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.21.6-158

Abstract

Background: The xenogeneic scaffold such as FDBB is considered immunogenic due to the presence of xenogeneic cell residue. A decellularization process is added to FDBB to reduce the inflammatory response. Objectives: The objective of this study is to evaluate the efficacy of dc-FDBB in reducing the host inflammatory response after implantation, compared to DBBM as the gold standard scaffold with renowned biocompatibility. Method: Sixty Rattus Novergicus were divided into four groups, subcutaneously implanted FDBB, dc-FDBB, DBBM scaffolds and one group is sham-operated. On the 2nd, 7th and 14th postoperative days, a thoracotomy was performed and 1.5 ml of intra-cardiac blood was taken for ELISA test. Statistical tests were carried out by testing the normality of the data (Shapiro-Wilk) and homogeneity (Levene), then a comparative test was carried out with the Analysis of Variance (ANOVA) on data that was normally distributed and homogeneous, on data that was not normally distributed or not homogeneous, a comparison test with the Kruskall test Wallis, followed by the Post Hoc Tuckey HSD test (p<0.05). Results: Statistical analysis on days 2, 7 and 14 on levels of IL-6 (p = 0.530, p = 0.279, p = 0.790), TNF-α (p = 0.250, p = 0.009, p = 0.324), and IL -10 (p = 0.743, p = 0.349, p = 0.374 as a whole showed the result (p>0.05), there was no significant difference between all groups. Results of analysis of TNF-α day 7 (p=0.009) with (p > 0.05) showed that there was a difference, and continued with the post hoc test with the result p = 0.005, there was a difference between the controls compared to the dc-FDBB. Conclusion: There was no significant difference in serum levels of IL-6, TNF-α, and IL-10 after FDBB and dcFDBB implantation.
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