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EFFECT OF DNA GC-CONTENT ON INTERACTION WITH METHYLENE BLUE

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CC BY-NC 4.0 This work is licensed under Creative Commons Attribution–NonCommercial International License (CC BY-NC 4.0).

Abstract

The melting of methylene blue (MB) complexes of DNA with different GC-content sequences at 0.02 M ionic strength of the solution has been carried out. It was shown that the change of melting interval width of MB complexes with DNA increases depending on GC-content enhancement within DNA sequences. It was revealed that MB is bound to DNA by semi-intercalation mode.

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