2015; Chemical and Biological Sciences 49 (2 (237): 40–44
Shared with The Gufo

ERROR IN DETERMINATION OF BINDING THERMODYNAMIC PARAMETERS APPEARED DUE TO ADSORPTION LINEAR ISOTHERM DESCRIPTION

S.N. Hakobyan*

Received: 2025-03-10 · Published: 2015-07-10

© 2015 The Author(s) and the original publisher.

Licence: as stated by the publisher.
Shared article.
Original title
ERROR IN DETERMINATION OF BINDING THERMODYNAMIC PARAMETERS APPEARED DUE TO ADSORPTION LINEAR ISOTHERM DESCRIPTION
Author
S.N. Hakobyan
Published
2015-07-10
Licence
as stated by the publisher

Abstract

In the present work the description of absorption spectra of ethidium bromide complexes with synthesized polyribonucleotide by several modes has been carried out. The binding constant value and the values of changes of thermodynamic parameters were determined. It was shown that at the description by linear binding isotherm the value of enthalpy and the number of nucleotides corresponding to one ligand molecule at interaction saturation are approximately the same as real ones, but the values of the binding constant, Gibbs free energy change and entropy change differ from real values.
1 / ? 100% Open in new tab Download Cite

Loading the full text…

Download Follow Updates