Bulletin of Stomatology and Maxillofacial Surgery
ISSN 1829-006X
2025; 250–258
Shared with The Gufo

IMPACT OF BOSINTHETICALY DERIVED β-CHITOSAN TITANIUM NANOPARTICLES ON THE ENHANCMENT OF CAUDAL FIN REGENERATION ZEBRAFISH MODELS

Isabel Benny*, Karthik Mohanraj, Taniya Martin, Meenakshi Sundaram Kishore Kumar

Department of Anatomy, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Poonamalle High Road, Velappanchavadi, Chennai-600077, Tamil Nadu, India.

Received: 2026-04-08 · Published: 2025-04-30

© 2025 The Author(s) and the original publisher.

CC BY-NC 4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Shared article.
Original title
IMPACT OF BOSINTHETICALY DERIVED β-CHITOSAN TITANIUM NANOPARTICLES ON THE ENHANCMENT OF CAUDAL FIN REGENERATION ZEBRAFISH MODELS
Authors
Isabel Benny, Karthik Mohanraj, Taniya Martin, Meenakshi Sundaram Kishore Kumar
Source journal
Bulletin of Stomatology and Maxillofacial Surgery
Published
2025-04-30
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.58240/1829006X-2025.3-250

Abstract

Background: Tissue regeneration is a crucial biological process with significant implications for developmental biology and regenerative medicine. Zebrafish (Danio rerio), known for their remarkable regenerative abilities, serve as an excellent model for studying tissue regeneration, particularly through the caudal fin regeneration model. Materials and Methods: β-Chitosan-TiO₂ nanoparticles were biosynthesized using plant extracts and microbial cultures. Characterization was performed using SEM, UV-Vis and FTIR. Zebrafish caudal fin regeneration was assessed post-amputation, with nanoparticle treatment in 16 μg/L and 32 μg/L. Regeneration was analyzed via microscopic observation and gene expression. Data were statistically analyzed using ANOVA, considering p < 0.05 significant. Results: β-Chitosan-TiO₂ nanoparticles exhibited uniform size, high stability, and strong biocompatibility. Zebrafish treated with β-Chitosan-TiO₂ nanoparticles showed significantly enhanced caudal fin regeneration compared to controls. Gene expression analysis supported upregulation of regenerative markers. Regenerated fin length measurements showed a dose-dependent improvement, with optimal effects at moderate nanoparticle concentrations. No significant toxicity or abnormalities were observed in treated zebrafish. Statistical analysis confirmed significant differences (p < 0.05) between control and treated groups, demonstrating β-Chitosan-TiO₂ NPs' potential in tissue regeneration. Conclusion: β-Chitosan-TiO₂ nanoparticles significantly enhanced zebrafish caudal fin regeneration by promoting cell proliferation and angiogenesis without toxicity. These findings highlight their potential as a biocompatible and eco-friendly nanomaterial for tissue regeneration applications.
1 / ? 100% Open in new tab Download Cite

Loading the full text…

Download Follow Updates