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    Author(s)

    M.V. Minasyants
    Category: Biology
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      Natural Science, Biology, 2025

      INVESTIGATION OF DIFFERENTIAL ABSORPTION OF DNA COMPLEXES WITH LIGANDS

      M.V. Minasyants
      This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

      © 2025 by author(s) and The Gufo Inc.
      CC BY-NC 4.0 This work is licensed under Creative Commons Attribution–NonCommercial International License (CC BY-NC 4.0).

      Abstract

      The investigation of complexes of ethidium bromide (EtBr) and methylene blue (MB) with DNA at 0.02 M Na+ ionic strength of solution has been carried out. Absorption and differential absorption spectra of EtBr and MB and their complexes with DNA were obtained. The isobestic point was revealed on the absorption and differential absorption spectra of EtBr and its complexes with DNA, while on analogous spectra of MB and its complexes with DNA there is no isobestic point.

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