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In the article influence of Montivipera raddei and Macrovipera lebetina obtusa venom including phospholipase A2 (PLA2) inhibited by p-bromophenacyl bromide on giant unilamellar vesicles, visualized with 8-anilino-1-naphthalenesulfonic acid fluorescence probe, was investigated. The results have shown that both venoms even without the PLA2 activity have a noticeable membranotropic effect on the artificial membranes, but in different ways, so one can propose that investigated viper venoms can contain different PLA2 isomers.