This work is licensed under Creative Commons Attribution–NonCommercial International License
(CC BY-NC 4.0).
The unique organization of lichen thallus provides still unexplored environment for microbial communities. The study aimed to isolate and identify endolichenic non photosynthetic diazotrophic bacteria from the thallus of corticolous lichen species Physcia biziana. Two strains of chemoorganoheterotrophic endospore-forming bacteria were isolated and identified based on 16S rRNA gene sequence analysis as Paenibacilluspolymyxa(>93.54% similarity). Characterization of cultivable strains suggest the involvement of associated bacteria in nitrogen cycling. This study highlights the significance of studying the endobacteriobiomeof lichens, as it is an important aspect of their multicomponent natural biofilm