2013; Chemical and Biological Sciences 47 (3 (232): 24–27
Shared with The Gufo

ROLE OF FUNCTIONAL SH GROUPS ON THE ACTIVITY EXPRESSION AND CONFORMATION MAINTENANCE OF RANARIDIBUNDAFROG LIVER ARGINASE

A.S. Shamirian*

Received: 2025-03-12 · Published: 2013-11-15

© 2013 The Author(s) and the original publisher.

Licence: as stated by the publisher.
Shared article.
Original title
ROLE OF FUNCTIONAL SH GROUPS ON THE ACTIVITY EXPRESSION AND CONFORMATION MAINTENANCE OF RANARIDIBUNDAFROG LIVER ARGINASE
Author
A.S. Shamirian
Published
2013-11-15
Licence
as stated by the publisher

Abstract

After inactivation by ethylenediaminetetraacetic acid (EDTA) the subunits (dimer, monomer) of Rana ridibunda frog’s liver arginase differs by sensitivity towards thiol reagents. Moreover, the particular activation of subunits is also observed at the presence of p-chloromercuribenzoate (p-CMB). It is notable, that p-CMB significantly decreases the activity of reactivated enzyme in the presence of Mn2+ ions, despite the fact that in native extracts the enzyme is not inhibited by the thiol reagent. Here we discussed the effect of p-CMB in the inactivation process not only as a result of SH groups’ modification, but also because of structural changes in native conformation by EDTA and non specific reaction of p-CMB with other functional groups.
1 / ? 100% Open in new tab Download Cite

Loading the full text…

Download Follow Updates