2023; Chemical and Biological Sciences 57 (3 (262): 214–221
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SPECTROPHOTOMETRIC STUDY OF COMPLEXES OF SOME INTERCALATORS WITH DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS

Ara Antonyan*, Zvart O. Movsisyan, Anzhela H. Karapetyan, Poghos H. Vardevanyan

Received: 2025-03-03 · Published: 2023-10-05

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Original title
SPECTROPHOTOMETRIC STUDY OF COMPLEXES OF SOME INTERCALATORS WITH DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS
Authors
Ara Antonyan, Zvart O. Movsisyan, Anzhela H. Karapetyan, Poghos H. Vardevanyan
Published
2023-10-05
Licence
Creative Commons Attribution-NonCommercial 4.0 International
Original
https://doi.org/10.46991/PYSU:B/2023.57.3.214

Abstract

Interaction of ethidium bromide (EtBr), acridine orange (AO) and non-intercalator methylene blue (MB) with DNA as well as interaction of EtBr and MB with single-stranded synthetic polynucleotides poly(rA) and poly(rU) have been studied. It was revealed that in absorption spectra of EtBr and MB with DNA or single-stranded polynucleotides a real- or pseudo-isosbestic point appears. Particularly, in absorption spectra of the complexes of classical intercalator EtBr with DNA the real isosbestic point is formed, but in the case of non-classical intercalator MB a pseudo-isosbestic point is formed, while in the absorption spectra of their complexes with single-stranded polynucleotides a pseudo-isosbestic point is formed. It was found out that for another classical intercalator AO there is no a real or pseudo-isosbestic point.
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