Abstract

Using DNA banks or gene libraries is the most efficient method of protecting and managing valuable, endangered, and rare gene pools. Genomic libraries are collections of bacteria colonies with restriction sites on their genomic DNA that contain desired genes from a specific organism. The “Scientific Center of Agro- Biotechnology” branch of ANAU has created gene libraries of 34 plant species, which are preserved in the National Genebank of Crops and their Wild Relatives, which forms a basis for genomic selection. This allows genetic identification, barcoding, DNA formula decoding, and, most importantly, is the basis for genome identification. Based on the results, plasmids can incorporate restriction segments of genomic DNA and be efficiently transformed. The high level of correlation between the number of necessary clones and the efficiency of transformation of all studied plant species proves the completeness of the gene pool.

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