2013; Physical and Mathematical Sciences, 47(3 (232): 57–63
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DETERMINATION OF MELTING TEMPERATURE FOR MULTI-PEAK DIFFERENTIAL MELTING CURVES OF DNA

Inessa Grigoryan*

Received: 2025-02-17 · Published: 2013-11-20

© 2013 The Author(s) and the original publisher.

CC BY 4.0 This work is licensed under a Creative Commons Attribution 4.0 International License.
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Original title
DETERMINATION OF MELTING TEMPERATURE FOR MULTI-PEAK DIFFERENTIAL MELTING CURVES OF DNA
Author
Inessa Grigoryan
Published
2013-11-20
Licence
Creative Commons Attribution 4.0 International

Abstract

This study was carried out because of a widely spread mistaken viewpoint that melting temperature (Tm) cannot be used as a reasonable parameter in the case of multi-peak differential melting curves (DMC) of DNA. However, there were no theoretical or experimental evidences or disclaimers of this viewpoint. In this work such study has been carried out. We have tested various definitions of Tm. Indeed, some of them give unreasonable dependences of Tm on relative concentration of modifications (rb). At same time, the average temperature of the helix-coil transition calculated as the integral over the temperature of the product of DMC and temperature gives reasonable smooth dependences Tm(rb). The same definition is the best for the dependence of Tm on the average GC-content.
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